N, N'-methylenebisacrylamide crosslink Initiator and Catalyst (NH4hS,OS/TEME NH2 NH Figure 12.2 Polymerization of acrylamide. Reproduced with permission from Bio-Rad Laboratories How Do You control pore size? Pore size is most efficiently and predictably regulated by manip- ulating the concentration of acrylamide in the gel. Pore size will ge with the amount of crosslinker but the effect is minimal crosslinker usually present in gels(2.7-5%) Practical experience with various ratios of acrylamide bis shown that it is best to change pore size by changing the 339How Do You Control Pore Size? Pore size is most efficiently and predictably regulated by manip￾ulating the concentration of acrylamide in the gel. Pore size will change with the amount of crosslinker, but the effect is minimal and less predictable (Figure 12.3). Note the greater impact of acry￾lamide concentration on pore size, especially at the levels of crosslinker usually present in gels (2.7–5%). Practical experience with various ratios of acrylamide:bis have shown that it is best to change pore size by changing the acry￾Electrophoresis 339 Figure 12.2 Polymerization of acrylamide. Reproduced with permission from Bio-Rad Laboratories