BEH.462/3. 962J Molecular Principles of Biomaterials Spring 2003 Primary =髻B second ad Figure 2. Large (c) he effertive potential /eXperiencer hy interaction potential between the bare surface and the protel Wigu re je smell ar tesr s all co can rat itra ter n ertion mechanism favors primary adsorption at the o Experimental measurements and comparison with theory o Measurements made using surface plasmon resonance o Experimental details o=on gr PEG MW= 2000 g/mole(N= 45) Layer thicknesses Lo=40-60 A of tested pre BPTI(bovine pancreatic trypsin inhibitor): MW=6000-g/mole;R-21x21x30 A HSA(human serum albumin ) MW=66, 200 g/mole; R=38X38x150 A FBN(Fibrinogen): MW=340,000 g/mole: R-55x55x460 A rod-link protein Comparison of theory stance between grafting sites, A with experiment FIGURE 9 lymer- Distance between grafting sites, A FIGURE 7: Characteristic time course for fibrinogen adsorption onto a supported DSPE monoLayer measured by SE FIaURE 10 on of HsA (D and FN ( o)as race pol ymer-lp (Efremova et al. 2000) Lecture15-‘ stealth’ particles 4 of 8BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 Lecture 15 – ‘stealth’ particles 4 of 8 (Halperin 1999) Primary adsorption secondary adsorption o Experimental measurements and comparison with theory o Measurements made using surface plasmon resonance6 o Experimental details: ƒ σ = on graphs! [area/chain] ƒ PEG MW = 2000 g/mole (N = 45) ƒ Layer thicknesses L0 = 40-60 Å ƒ Sizes of tested proteins: • BPTI (bovine pancreatic trypsin inhibitor) : MW = 6000- g/mole; R ~ 21x21x30 Å • HSA (human serum albumin): MW = 66,200 g/mole; R = 38x38x150 Å • FBN (Fibrinogen): MW = 340,000 g/mole; R ~ 55x55x460 Å rod-link protein Comparison of theory with experiment (Efremova et al. 2000)