solation of fungal pathogens from infected plant tissue Infected plant Sections from margin of Sterile forceps Tissue sections blotted Sections are lesion placed in I0% clorox used to transfer with sterile paper placed on for different durations sections towel to rernove nutrient clorox excess medium in petri dish E留,燃 口 Sechons placed on In correct immersion A pure culture of the pathogen is obtained by nutient media in leg.90 )only the subculturing a segment of the pathogengrowth arder of immersion pathogen survives in in the previous plate into a new plate with time in clorox center of section and nutr ient medium grows out of the tissue