Cell B c RT RT transplan 日cold+Abx 目- Time(hrs) G“門" od transplanted RT transplanted _ Colon 6 GF RT Cold donors 碳 Small Duodenum Duodenum emeter 且团RT+Ab Cold +Abx 18 M 1 F- WN 吻 E ON FI s Cold Small Colon ntestine Figure 5. Cold-Exposed and Cold Microbiota- Transplanted Mice Show Increased Intestinal Length and Caloric Uptake (A and B) Oral glucose tolerance test (oGTT)of cold-exposed mice with or without Abx treatment (A, or RT- and cold microbiota-transplanted mice 16 days after transplantation( B)(n=8 per group) (egend continued on next page) ce163,1360-1374, December3,2015@2015 Elsevier Inc.1367Weight (g) Small intestine Colon Stomach Cecum *** 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 RT RT+Abx Cold Cold+Abx Small intestine Colon Length (cm) 5 6 7 8 9 35 40 45 50 [***] Length (cm) Small intestine Colon *** 5 6 7 8 9 34 36 38 40 42 * RT donors Cold donors 6 8 10 12 34 36 38 40 42 44 Length (cm) RT transplanted Cold transplanted Germ free Small intestine Colon *** *** RT Cold Cold+Abx Cold GF RT+Abx RT GF 5 6 7 8 9 36 40 44 48 Small intestine Colon GF RT GF Cold Length (cm) *** 0 30 60 90 120 15 5 10 15 20 25 RT RT + Abx Cold Cold + Abx 024 1 20 40 60 80 100 024 1 0.3 0.6 0.9 * ** * RT transplanted Cold transplanted * p = 0.1 RT transplanted Cold transplanted * * 0 30 60 90 120 5 10 15 20 25 RT transplanted Cold transplanted * 0 1 0 2 0 3 0 4 0 Kcal uptake per 24 hrs * Cold RT 500µm 200µm RT RT+Abx Cold Cold+Abx Duodenum perimeter Duodenum villi 0 1 0 2 0 3 0 Cold transplanted RT transplanted * RT transplanted Cold transplanted RT RT+Abx Cold Cold+Abx Duodenum perimeter (µm) 2000 4000 6000 8000 10000 [**] 0 5 00 1 00 0 1 50 0 Duodenum villi length (µm) *** ** *** A BC D E FG H I J K L MN O P * * * * ** *** * *** *** OGTT - Glycemia (mM) OGTT - Glycemia (mM) Time (hrs) Time (hrs) Time (hrs) Time (hrs) Triglycerides (mg / dl) Free fatty acids (mM) Kcal uptake per 24 hrs Figure 5. Cold-Exposed and Cold Microbiota-Transplanted Mice Show Increased Intestinal Length and Caloric Uptake (A and B) Oral glucose tolerance test (OGTT) of cold-exposed mice with or without Abx treatment (A), or RT- and cold microbiota-transplanted mice 16 days after transplantation (B) (n = 8 per group). (legend continued on next page) Cell 163, 1360–1374, December 3, 2015 ª2015 Elsevier Inc. 1367