1. Target selection(Grouping The targets for structure determination will be 1.Individual domains rather than multi-domain proteins(Chris Sander, Millenium Information 2. Easier to determine by X-ray crystallography or NMR spectroscopy than the more flexible multi domain proteins 3. Done by pairwise comparison of all protein sequences, followed by clustering into groups of domains 4. The representatives of the groups without structurally defined members 5. Members that share at least 30% sequence identity (30-seq families), rather than those that correspond to superfamiles or fold families 0/27/2005 Chaoqun Wu, Fudan University10/27/2005 Chaoqun Wu, Fudan University Chaoqun Wu, Fudan University 9 1. Target selection (Grouping) The targets for structure determination will be 1. Individual domains rather than multi-domain proteins (Chris Sander, Millenium Information). 2. Easier to determine by X-ray crystallography or NMR spectroscopy than the more flexible multi￾domain proteins. 3. Done by pairwise comparison of all protein sequences, followed by clustering into groups of domains. 4. The representatives of the groups without structurally defined members. 5. Members that share at least 30% sequence identity (30-seq families), rather than those that correspond to superfamiles or fold families. The targets for structure determination will be 1. Individual domains rather than multi-domain proteins (Chris Sander, Millenium Information). 2. Easier to determine by X-ray crystallography or NMR spectroscopy than the more flexible multi￾domain proteins. 3. Done by pairwise comparison of all protein sequences, followed by clustering into groups of domains. 4. The representatives of the groups without structurally defined members. 5. Members that share at least 30% sequence identity (30-seq families), rather than those that correspond to superfamiles or fold families