The PCR cycle 95C step to denature the duplex dna An annealing step of around 55c to allow the primers to bind 72C polymerization step g2+, dNTPs are required in addition to template, primers, buffer and enzymeThe PCR cycle • 95C step to denature the duplex DNA, • An annealing step of around 55C to allow the primers to bind, • 72C polymerization step. • Mg2+,dNTPs are required in addition to template,primers,buffer and enzyme