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Question 6 you have a strain of yeast that glows purple when compound a is added to the medium you suspect that compound A is a precursor of the " glowing"pathway. you mutagenize the original haploid strain and isolate six mutants that do not glow purple in the presence of compound A Since haploid yeast can be mated to make diploids, you mate each mutant strain with your complement each others' phenotypes. The results are summarized below ta ominant or original WT purple yeast to determine whether the mutant phenotypes are dominant or recessive you also mate each mutant strain with each other to see which strains can C+means colonies glow purpl means they do not glow purple) Strains m1 WT Mated + a)Are all the mutant phenotypes recessive? es. No. 2 pts If no, indicate the mutants having dominant phenotypes b)Place the mutants into complementation groups. 6 pts m1 m2 m6 m3 m5 c)How many genes have you identified that are involved in this pathway? 2 ptsQuestion 6 You have a strain of yeast that glows purple when compound A is added to the medium. You suspect that compound A is a precursor of the “glowing” pathway. You mutagenize the original haploid strain and isolate six mutants that do not glow purple in the presence of compound A. Since haploid yeast can be mated to make diploids, you mate each mutant strain with your original WT purple yeast to determine whether the mutant phenotypes are dominant or recessive. You also mate each mutant strain with each other to see which strains can complement each others’ phenotypes. The results are summarized below. (“+” means colonies glow purple, “-“ means they do not glow purple) Strains Mated m1 m2 m3 m4 m5 m6 WT m1 - - + + + - + m2 - + + + - + m3 - + - + + m4 - + + + m5 - + + m6 - + a) Are all the mutant phenotypes recessive? Yes. No. 2 pts. If no, indicate the mutants having dominant phenotypes. b) Place the mutants into complementation groups. 6 pts m1 m2 m6 m3 m5 m4 c) How many genes have you identified that are involved in this pathway? 2 pts 10 3
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