Secondary syphilis Treponema pallidum spread in the bloodstream and reach their highest numbers Appearance 6 to 8 weeks after the development of the chancre or 3 to 4 weeks after the healing of the chancre.Secondary syphilis • Treponema pallidum spread in the bloodstream and reach their highest numbers • Appearance 6 to 8 weeks after the development of the chancre or 3 to 4 weeks after the healing of the chancre