B Techniques for studying the cytoskeleton o Fluorescent microscopy and Electron microscopy: Immunofluorescence: fluorescently-labeled antibod Fluorescence: microinject into living cells Video microscopy: in vitro motility assays Electron: Triton X-100, Metal replica Quick freezing-deep etching EM Biochemical analysis (in vitro Difference centrifugation; SDS-PAGE 4 Drugs and mutations(about functionsB. Techniques for studying the cytoskeleton ❖ Fluorescent microscopy and Electron microscopy : Immunofluorescence: fluorescently-labeled antibody Fluorescence: microinject into living cells Video microscopy: in vitro motility assays Electron: Triton X-100, Metal replica Quick freezing-deep etching EM ❖ Biochemical analysis (in vitro) Difference centrifugation; SDS-PAGE ❖ Drugs and mutations (about functions)