Contents Preface Contributors Chapter I. Preparing for Success in the Laboratory Phillip P. Franciskovich Chapter 2. Getting What You Need from a Supplier Tom Tyre and greg Krueger Chapter 3. The Preparation of Buffers and other Solutions: A Chemists Perspective Edward A. Pfannkoch Chapter 4. How to Properly Use and Maintain Laboratory Trevor troutman Kristin a. Prasauckas. michele A Kennedy, Jane Stevens, Michael G. Davies, and Andrew T Dadd Chapter 5. Working Safely with Biological Samples Constantine G. Haidaris and Eartell ]. Brownlow Chapter 6. Working Safely with Radioactive Materials William R.. Volny ]r Chapter 7. DNA Purification 167 Chapter 8. RNA Purification ori A. Martin, Tiffany. Smith, Dawn Obermoeller Brian Bruner: Martin Kracklauer and Subramanian Dharmarajvii Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xi Chapter 1. Preparing for Success in the Laboratory Phillip P. Franciskovich . . . . . . . . . . . . . . . . . . . . . . . . 1 Chapter 2. Getting What You Need from a Supplier Tom Tyre and Greg Krueger . . . . . . . . . . . . . . . . . . . . 11 Chapter 3. The Preparation of Buffers and Other Solutions: A Chemist’s Perspective Edward A. Pfannkoch . . . . . . . . . . . . . . . . . . . . . . . . . 31 Chapter 4. How to Properly Use and Maintain Laboratory Equipment Trevor Troutman, Kristin A. Prasauckas, Michele A. Kennedy, Jane Stevens, Michael G. Davies, and Andrew T. Dadd . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49 Chapter 5. Working Safely with Biological Samples Constantine G. Haidaris and Eartell J. Brownlow . . . . . 113 Chapter 6. Working Safely with Radioactive Materials William R. J. Volny Jr. . . . . . . . . . . . . . . . . . . . . . . . . . 141 Chapter 7. DNA Purification Sibylle Herzer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 167 Chapter 8. RNA Purification Lori A. Martin, Tiffany J. Smith, Dawn Obermoeller, Brian Bruner, Martin Kracklauer, and Subramanian Dharmaraj . . . . . . . . . . . . . . . . . . . . . . . 197 Contents