(3) A regulated bifunctional enzyme( PFK-2 and FBPase-2)synthesizes(from Fru-6-P)and degrades fructose 2, 6-bisphosphate A feedforward stimulation Fru-6-P stimulate the synthesis and inhibits the hydrolysis of Fru-2, 6-bisphosphate, which in turn stimulates pfk-1 O O—P-O—CH2O、CH2OH O H HO H O-P-0 OH H O Fructose 2, 6-bisphosphate(3) A regulated bifunctional enzyme (PFK-2 and FBPase-2) synthesizes (from Fru-6-P) and degrades fructose 2,6-bisphosphate. A feedforward stimulation: Fru-6-P stimulate the synthesis and inhibits the hydrolysis of Fru-2,6-bisphosphate, which in turn stimulates PFK-1