2. Intermembrane space( perinuclear space) The Intermembrane space is one by that two nuclear membranes are separated with 10-50 nm distance. The perinuclear space is directly connected with the lumen of the er. The perinuclear space is as a functionally physiological buffer zone. The perinuclear space is sensitive to pathological changes of the cell 3. Nuclear pore complex (NPC) The inner and outer nuclear membrane are joined at sites forming circle pores, called the nuclear pore complex(nPC). The average mammalian cell contains approximately 3000 nuclear pores. (see the next section)2. Intermembrane space ( perinuclear space) The Intermembrane space is one by that two nuclear membranes are separated with 10-50 nm distance. The perinuclear space is directly connected with the lumen of the ER. The perinuclear space is as a functionally physiological buffer zone.The perinuclear space is sensitive to pathological changes of the cell. 3.Nuclear pore complex (NPC) The inner and outer nuclear membrane are joined at sites forming circle pores, called the nuclear pore complex (NPC). The average mammalian cell contains approximately 3000 nuclear pores.(see the next section)