5 site 3 site 22.3 Nuclear splicing GU UACUAAC AG proceeds through a lariat y8ON PY80 PY87 Pu75 A Ry95 Animal consensus Cut at 5 site &form lariat by 5-2 bond conne cting the intron 5 -G to the 2 'of a at the branch site Figure 22.6 Splicing occurs in UACUAACAG two stages. in Cut at 3 site join exons: intron re le ased as lariat which the5￠ VACUA exon is separated an en IS joined to the3￠exon Debranch introl 消当 UACUAAC AFigure 22.6 Splicing occurs in two stages, in which the 5￠ exon is separated and then is joined to the 3￠ exon. 22.3 Nuclear splicing proceeds through a lariat