3. The multimeric RNA polymerase in E, coli has multiple functions The holoenzyme consists of five types of subunits (a2BBo)and its is used to synthesize all the rna molecules in e coli The multiple functions include searches for initiation sites on the dna molecule and unwinds a short stretch of DNa(initiation) selects the correct NTP and catalyzes the formation of phosphodiester bonds(elongation) detects termination signals for RNa synthesis (termination)3. The multimeric RNA polymerase in E.coli has multiple functions ⚫ The holoenzyme consists of five types of subunits (a2bb’ s)and its is used to synthesize all the RNA molecules in E. coli. ⚫ The multiple functions include: – searches for initiation sites on the DNA molecule and unwinds a short stretch of DNA (initiation); – selects the correct NTP and catalyzes the formation of phosphodiester bonds (elongation); – detects termination signals for RNA synthesis (termination)