44 高分子通报 2013年8月 [24 Weiner S, Wagner H D. Annu Rev Mater Sci. 1998, 28(1):271-298. 25] Giraud-Guille MM. Calcif Tissue Int, 1988, 42(3):167-180 S Giraud-Guille MM, Besseau L, Martin R. J Biomech, 2003, 36(10): 1571-1579 [271 Mosser G, Anglo A, Helary C, Bouligand Y, Giraud-Guille MM. Matrix Biol, 2006, 25(1):3-13. [29] Cowin S C. J Non-Newtonian Fluid Mech, 2004, 119(1): 155-162. [30] Gobeaux F, Mosser G, Anglo A, Panine P, Davidson P, Giraud-Guille MM, Belamie E. J Mol Biol, 2008, 376(5): 1509--1522. [31] Giraud Guille MM, Mosser G, Helary C, Eglin D. Micron, 2005, 36(7): 602-608 [32] Eglin D, Mosser G, GiraudGuille MM, Livage J, Coradin T. Soft Matter, 2005, 1(2):129-131. Progress on Structures of Supermolecular Collagenous Liquid Crystal in Animal Connective Tissue LI LHli, CHEN Lu, LI Xia, SUN Peng.2., HAO Na, JIANG Bo' (l National Engineering Research Center for Biomaterials, Engineering Research Center in Biomaterials, Sichuan University, Chengdu 610064, Chin 2. Key laboratory for Radiation Physics and Technology of the Ministry of Education, Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610064, China; 3. De partment of Mathematics and Physics, Officers College of CAPF, Chengdu 610213, China) Abstract: The occurrence, development and pathological changes of animal connective tissue are closely related to their structures and morphologies, and particularly the orderliness on the structure of tissue is apparent, liquid crystalline structures of collagen in connective tissue are a transition state that collagen molecules self-assemble into collagen fibers or tissue. Based on reconstituted collagen molecules, we have discussed that triple helical structure has an impact on collagen molecules assembling into liquid crystal, and have analyzed research progress on structural transformation of collagen molecular liquid crystal and super-molecular liquid crystal, as well as thermal and dynamic forming conditions. Inducing the formation of collagen liquid crystal is the key to simulate construction of the extracellular matrix in vitro. It not only established contact for studying the mechanisms of connective tissue morphogenesis and pathological process, but also provided useful information for building tissue engineering scaffolds. Key words: Collagen molecules: Entropy induced order: Molecular liquid crystal: Super molecular liquid crystal Connective tisst C1994-2013ChinaAcademicJOurnalElectronicPublishingHouse.Allrightsreservedhttp://www.cnki.net［２４］ ＷｅｉｎｅｒＳ，ＷａｇｎｅｒＨ Ｄ．ＡｎｎｕＲｅｖＭａｔｅｒＳｃｉ，１９９８，２８（１）：２７１～２９８． ［２５］ Ｇｉｒａｕｄ－ＧｕｉｌｌｅＭ Ｍ．ＣａｌｃｉｆＴｉｓｓｕｅＩｎｔ，１９８８，４２（３）：１６７～１８０． ［２６］ Ｇｉｒａｕｄ－ＧｕｉｌｌｅＭ Ｍ，ＢｅｓｓｅａｕＬ，ＭａｒｔｉｎＲ．ＪＢｉｏｍｅｃｈ，２００３，３６（１０）：１５７１～１５７９． ［２７］ ＭｏｓｓｅｒＧ，ＡｎｇｌｏＡ，ＨｅｌａｒｙＣ，ＢｏｕｌｉｇａｎｄＹ，Ｇｉｒａｕｄ－ＧｕｉｌｌｅＭ Ｍ．ＭａｔｒｉｘＢｉｏｌ，２００６，２５（１）：３～１３． ［２８］ ＰａｒｋｉｎｓｏｎＪ，ＫａｄｌｅｒＫＥ，ＢｒａｓｓＡ．ＪＭｏｌＢｉｏｌ，１９９５，２４７（４）：８２３～８３１． ［２９］ ＣｏｗｉｎＳＣ．ＪＮｏｎ－ＮｅｗｔｏｎｉａｎＦｌｕｉｄＭｅｃｈ，２００４，１１９（１）：１５５～１６２． ［３０］ ＧｏｂｅａｕｘＦ，ＭｏｓｓｅｒＧ，ＡｎｇｌｏＡ，ＰａｎｉｎｅＰ，ＤａｖｉｄｓｏｎＰ，Ｇｉｒａｕｄ－ＧｕｉｌｌｅＭ Ｍ，ＢｅｌａｍｉｅＥ．ＪＭｏｌＢｉｏｌ，２００８，３７６（５）：１５０９～１５２２． ［３１］ ＧｉｒａｕｄＧｕｉｌｌｅＭ Ｍ，ＭｏｓｓｅｒＧ，ＨｅｌａｒｙＣ，ＥｇｌｉｎＤ．Ｍｉｃｒｏｎ，２００５，３６（７）：６０２～６０８． ［３２］ ＥｇｌｉｎＤ，ＭｏｓｓｅｒＧ，Ｇｉｒａｕｄ－ＧｕｉｌｌｅＭ Ｍ，ＬｉｖａｇｅＪ，ＣｏｒａｄｉｎＴ．ＳｏｆｔＭａｔｔｅｒ，２００５，１（２）：１２９～１３１． ＰｒｏｇｒｅｓｓｏｎＳｔｒｕｃｔｕｒｅｓｏｆＳｕｐｅｒｍｏｌｅｃｕｌａｒＣｏｌｌａｇｅｎｏｕｓＬｉｑｕｉｄ ＣｒｙｓｔａｌｉｎＡｎｉｍａｌＣｏｎｎｅｃｔｉｖｅＴｉｓｓｕｅ ＬＩＬｉ－ｌｉ１，ＣＨＥＮＬｕ１，ＬＩＸｉａ１，ＳＵＮＰｅｎｇ１，２，３，ＨＡＯ Ｎａ１，ＪＩＡＮＧＢｏ１＊ （１．ＮａｔｉｏｎａｌＥｎｇｉｎｅｅｒｉｎｇＲｅｓｅａｒｃｈＣｅｎｔｅｒｆｏｒＢｉｏｍａｔｅｒｉａｌｓ，ＥｎｇｉｎｅｅｒｉｎｇＲｅｓｅａｒｃｈＣｅｎｔｅｒｉｎ Ｂｉｏｍａｔｅｒｉａｌｓ，ＳｉｃｈｕａｎＵｎｉｖｅｒｓｉｔｙ，Ｃｈｅｎｇｄｕ６１００６４，Ｃｈｉｎａ； ２．ＫｅｙＬａｂｏｒａｔｏｒｙｆｏｒＲａｄｉａｔｉｏｎＰｈｙｓｉｃｓａｎｄＴｅｃｈｎｏｌｏｇｙｏｆｔｈｅＭｉｎｉｓｔｒｙｏｆＥｄｕｃａｔｉｏｎ，ＩｎｓｔｉｔｕｔｅｏｆＮｕｃｌｅａｒ ＳｃｉｅｎｃｅａｎｄＴｅｃｈｎｏｌｏｇｙ，ＳｉｃｈｕａｎＵｎｉｖｅｒｓｉｔｙ，Ｃｈｅｎｇｄｕ６１００６４，Ｃｈｉｎａ； ３．ＤｅｐａｒｔｍｅｎｔｏｆＭａｔｈｅｍａｔｉｃｓａｎｄＰｈｙｓｉｃｓ，ＯｆｆｉｃｅｒｓＣｏｌｌｅｇｅｏｆＣＡＰＦ，Ｃｈｅｎｇｄｕ６１０２１３，Ｃｈｉｎａ） Ａｂｓｔｒａｃｔ：Ｔｈｅｏｃｃｕｒｒｅｎｃｅ，ｄｅｖｅｌｏｐｍｅｎｔａｎｄｐａｔｈｏｌｏｇｉｃａｌｃｈａｎｇｅｓｏｆａｎｉｍａｌｃｏｎｎｅｃｔｉｖｅｔｉｓｓｕｅａｒｅｃｌｏｓｅｌｙｒｅｌａｔｅｄｔｏｔｈｅｉｒ ｓｔｒｕｃｔｕｒｅｓａｎｄ ｍｏｒｐｈｏｌｏｇｉｅｓ，ａｎｄｐａｒｔｉｃｕｌａｒｌｙｔｈｅｏｒｄｅｒｌｉｎｅｓｓｏｎｔｈｅｓｔｒｕｃｔｕｒｅｏｆｔｉｓｓｕｅｉｓａｐｐａｒｅｎｔ，ｌｉｑｕｉｄｃｒｙｓｔａｌｌｉｎｅ ｓｔｒｕｃｔｕｒｅｓｏｆｃｏｌｌａｇｅｎｉｎｃｏｎｎｅｃｔｉｖｅｔｉｓｓｕｅａｒｅａｔｒａｎｓｉｔｉｏｎｓｔａｔｅｔｈａｔｃｏｌｌａｇｅｎｍｏｌｅｃｕｌｅｓｓｅｌｆ－ａｓｓｅｍｂｌｅｉｎｔｏｃｏｌｌａｇｅｎｆｉｂｅｒｓｏｒ ｔｉｓｓｕｅ．Ｂａｓｅｄｏｎｒｅｃｏｎｓｔｉｔｕｔｅｄｃｏｌｌａｇｅｎｍｏｌｅｃｕｌｅｓ，ｗｅｈａｖｅｄｉｓｃｕｓｓｅｄｔｈａｔｔｒｉｐｌｅｈｅｌｉｃａｌｓｔｒｕｃｔｕｒｅｈａｓａｎｉｍｐａｃｔｏｎｃｏｌｌａｇｅｎ ｍｏｌｅｃｕｌｅｓａｓｓｅｍｂｌｉｎｇｉｎｔｏｌｉｑｕｉｄｃｒｙｓｔａｌ，ａｎｄｈａｖｅａｎａｌｙｚｅｄｒｅｓｅａｒｃｈｐｒｏｇｒｅｓｓｏｎｓｔｒｕｃｔｕｒａｌｔｒａｎｓｆｏｒｍａｔｉｏｎｏｆｃｏｌｌａｇｅｎ ｍｏｌｅｃｕｌａｒｌｉｑｕｉｄｃｒｙｓｔａｌａｎｄｓｕｐｅｒ－ｍｏｌｅｃｕｌａｒｌｉｑｕｉｄｃｒｙｓｔａｌ，ａｓｗｅｌｌａｓｔｈｅｒｍａｌａｎｄｄｙｎａｍｉｃｆｏｒｍｉｎｇｃｏｎｄｉｔｉｏｎｓ．Ｉｎｄｕｃｉｎｇｔｈｅ ｆｏｒｍａｔｉｏｎｏｆｃｏｌｌａｇｅｎｌｉｑｕｉｄｃｒｙｓｔａｌｉｓｔｈｅｋｅｙｔｏｓｉｍｕｌａｔｅｃｏｎｓｔｒｕｃｔｉｏｎｏｆｔｈｅｅｘｔｒａｃｅｌｌｕｌａｒｍａｔｒｉｘｉｎｖｉｔｒｏ．Ｉｔｎｏｔｏｎｌｙ ｅｓｔａｂｌｉｓｈｅｄｃｏｎｔａｃｔｆｏｒｓｔｕｄｙｉｎｇｔｈｅ ｍｅｃｈａｎｉｓｍｓｏｆｃｏｎｎｅｃｔｉｖｅｔｉｓｓｕｅ ｍｏｒｐｈｏｇｅｎｅｓｉｓａｎｄｐａｔｈｏｌｏｇｉｃａｌｐｒｏｃｅｓｓ，ｂｕｔａｌｓｏ ｐｒｏｖｉｄｅｄｕｓｅｆｕｌｉｎｆｏｒｍａｔｉｏｎｆｏｒｂｕｉｌｄｉｎｇｔｉｓｓｕｅｅｎｇｉｎｅｅｒｉｎｇｓｃａｆｆｏｌｄｓ． Ｋｅｙｗｏｒｄｓ：Ｃｏｌｌａｇｅｎ ｍｏｌｅｃｕｌｅｓ；Ｅｎｔｒｏｐｙｉｎｄｕｃｅｄｏｒｄｅｒ；Ｍｏｌｅｃｕｌａｒｌｉｑｕｉｄｃｒｙｓｔａｌ；Ｓｕｐｅｒ－ｍｏｌｅｃｕｌａｒｌｉｑｕｉｄｃｒｙｓｔａｌ； Ｃｏｎｎｅｃｔｉｖｅｔｉｓｓｕｅ · ４４ · 高 分 子 通 报 ２０１３年８月