Genes Antibody Genes Antibody VL VH CHI CH3CH2 CHI Genes coding for human constant regions(CH,CL) and mouse variant regions(VH,VL)are combined Binds TNF Hybrid antibody 70%human,30%mouse Genes Human Mouse ▣ Bound,inactive TNF Genes coding for the hybrid antibody are transfected to E.coli and expanded ⑤ 人 Monoclonal "chimeric" antibodies to TNF are isolated