Profiling the Human gut Microbiota o Culture: the gold standard for identification of microbial species <30%of the gut microbiota has been cultured o Culture-independent techniques: offer a more representative snapshot' of the gut microbiota microbial diversity of the gut microbiota >Qualitative and quantitative information on bacterial species and their genetic capacity Changes in the gut microbiota in relation to perturbation or diseaseProfiling the Human Gut Microbiota Culture: the gold standard for identification of microbial species <30% of the gut microbiota has been cultured Culture-independent techniques: offer a more representative ‘snapshot’ of the gut microbiota Microbial diversity of the gut microbiota Qualitative and quantitative information on bacterial species and their genetic capacity Changes in the gut microbiota in relation to perturbation or disease