生物大分子波谐学原理 吴季辉 7.313℃一13℃相关谱 Q2B R74β0 E18 V26邱 161a 123a S57B 是 T14a T12a 00 8 0 F2=30.2,62.3ppm 5.0 4.0 3.0 2.0 1.0 F3(ppm) FIGURE 7.23 A selected F('H)-F(H)slice from a constant-time HCCH-COSY spectrum of C-labeled ubiquitin in D2O solution,acquired using the pulse se- quence illustrated in Fig.7.22b.Negative cross-peaks,which correspond to reso- nances that have been aliased in the F2(3C)dimension,are plotted with a single level only;these peaks have F2(13C)chemicals shifts of 62.3 ppm.The labels indicate the assignment in the F ('H)and F2(13C)dimensions.7.3 13C－13C相关谱 生物大分子波谱学原理 吴季辉