基因组学与应用生物学,2011年,第30卷,第6期,第748-758页 cs and Ap ology,2011,vol.30,No.6,748-75 评述与展望 Review and Progress DNA条形码研究进展 程希婷王爱民顾志峰王嫣战欣石耀华 热带生物资源教育部重点实验室,海南省热带水生生物技术重点实验室,海南大学海洋学院,海口,570228 通讯作者,sone70@126com 摘要DNA条形码是应用有足够变异的标准化短基因片段对物种进行快速、准确鉴定的新的生物身份识 别系统。2003年,加拿大 Guelph大学 Hebert等首次正式提出了DNA条形码概念,2004年成立了生物条形 码联盟,目前有来自50个国家的两百多个组织成为其成员,2007年5月加拿大 Guelph大学组建了世界上 第一个 dna barcoding鉴定中心,2009年1月正式启动“国际生命条形码计划”,中国科学院代表中国与加 拿大、美国和欧盟共同为iBOL4个中心节点。线粒体细胞色素C氧化酶基因COI具有引物通用性高和进 化速率快等优点,是理想的动物DNA条形码,不过,COⅠ在植物中应用效果较差,因此,核糖体IS序列和 质体tbcL、maK和tmH-psbA等序列也相继被引入植物的DNA条形码研究。虽然DNA条形码研究还处于 起步阶段,面临巨大挑战,但是,越来越多的研究表明DNA条形码可以广泛应用于生物的分类和鉴定,是 种简便、高效、准确的物种鉴定技术,已经在动物、植物和微生物等硏究中取得了显著成果,是生命科学领域 发展最快的学科前沿之一。本文从DNA条形码的开发、应用、国内相关文献研究现状、DNA条形码面临的挑 战以及发展前景等进行了综合分析,以期推动我国DNA条形码和分类学研究的发展。 关键词DNA条形码,分类学,物种鉴定 Current Progress of DNa barcoding Cheng Xiting Wang Aimin Gu Zhifeng Wang Yan Zhan Xin Shi Yaohua The Key Laboratory of Tropic Biological Resources, Minister of Education, Hainan Key Laboratory of Tropical Hydrobiology Technology, the Ocean College, Hainan University, Haikou, 570228 *Correspondingauthorstone70@126.com Ol:10.3969/gab.030000748 Abstract DNa barcoding is a new life identification system which can distinguish species rapidly and accurately by analyzing standard short DNA sequences with enough variation. In 2003, the concept of dNa barcoding was formally proposed by Hebert and his colleagues, Canada biologists from University of Guelph. In 2004, Consor- tium for the Barcode of Life(CBOL) was subsequently constructed. There are more than 200 group members from 50 different countries in CBOL. The first dNa barcode identifying center came into existence in the University of Guelph in May, 2007. In January, 2009, International Barcode of Life(iBOL) was started up. Chinese Academy of Sciences, deputy of China, was one of four subcenter of iBOL, the same as others in Canda, USA and European Union. Mitochondrion cytochrome c oxidase subunit I(co I) was ideal DNa barcoding sequence for animal because of its various advantages, such as high primer universality and evolutionary rate. However, coI was not so good dNA barcoding in plant as in animal. Thus, ribosome Internal Transcribed Spacer (ITS) and plastid rb- cL, matK, trnH-psbA sequences were used as DNA barcoding in plant studying. Although it is on the initial phase for DNA barcoding, facing great challenge, more and more studies showed that DNA barcoding can be widely used in life taxonomy and identification. DNA barcoding, a simple effective accurate species identification method, is now one of the fastest development discipline hot in biological study. Here, in order to promote devel- opment of Chinese study in DNA barcoding and taxonomy, we introduced DNA barcoding screening, application, 基金项目:本研究由国家科技支撑计划项目(2009BAB44B00)和海南大学“211工程”创新人才计划项目共同资助 91994-2012ChinaAcademicJournalElectronicPublishingHouse.Allrightsreservedhttp:/www.cnki.net基因组学与应用生物学，2011 年，第 30 卷，第 6 期，第 748-758 页 Genomics and Applied Biology, 2011, Vol.30, No.6, 748-758 评述与展望 Review and Progress DNA 条形码研究进展 程希婷 王爱民 顾志峰 王嫣 战欣 石耀华 * 热带生物资源教育部重点实验室, 海南省热带水生生物技术重点实验室, 海南大学海洋学院, 海口, 570228 * 通讯作者, stone70@126.com 摘 要 DNA 条形码是应用有足够变异的标准化短基因片段对物种进行快速、准确鉴定的新的生物身份识 别系统。2003 年，加拿大 Guelph 大学 Hebert 等首次正式提出了 DNA 条形码概念，2004 年成立了生物条形 码联盟，目前有来自 50 个国家的两百多个组织成为其成员，2007 年 5 月加拿大 Guelph 大学组建了世界上 第一个 DNA barcoding 鉴定中心，2009 年 1 月正式启动“国际生命条形码计划”，中国科学院代表中国与加 拿大、美国和欧盟共同为 iBOL 4 个中心节点。线粒体细胞色素 C 氧化酶基因 COⅠ具有引物通用性高和进 化速率快等优点，是理想的动物 DNA 条形码，不过，COⅠ在植物中应用效果较差，因此，核糖体 ITS 序列和 质体 rbcL、matK 和 trnH-psbA 等序列也相继被引入植物的 DNA 条形码研究。虽然 DNA 条形码研究还处于 起步阶段，面临巨大挑战，但是，越来越多的研究表明 DNA 条形码可以广泛应用于生物的分类和鉴定，是一 种简便、高效、准确的物种鉴定技术，已经在动物、植物和微生物等研究中取得了显著成果，是生命科学领域 发展最快的学科前沿之一。本文从 DNA 条形码的开发、应用、国内相关文献研究现状、DNA 条形码面临的挑 战以及发展前景等进行了综合分析，以期推动我国 DNA 条形码和分类学研究的发展。 关键词 DNA 条形码, 分类学, 物种鉴定 Current Progress of DNA Barcoding Cheng Xiting Wang Aimin Gu Zhifeng Wang Yan Zhan Xin Shi Yaohua * The Key Laboratory of Tropic Biological Resources, Minister of Education, Hainan Key Laboratory of Tropical Hydrobiology Technology, the Ocean College, Hainan University, Haikou, 570228 * Corresponding author, stone70@126.com DOI: 10.3969/gab.030.000748 Abstract DNA barcoding is a new life identification system which can distinguish species rapidly and accurately by analyzing standard short DNA sequences with enough variation. In 2003, the concept of DNA barcoding was formally proposed by Hebert and his colleagues, Canada biologists from University of Guelph. In 2004, Consor￾tium for the Barcode of Life (CBOL) was subsequently constructed. There are more than 200 group members from 50 different countries in CBOL. The first DNA barcode identifying center came into existence in the University of Guelph in May, 2007. In January, 2009, International Barcode of Life (iBOL) was started up. Chinese Academy of Sciences, deputy of China, was one of four subcenter of iBOL, the same as others in Canda, USA and European Union. Mitochondrion cytochrome c oxidase subunit Ⅰ(COⅠ) was ideal DNA barcoding sequence for animal because of its various advantages, such as high primer universality and evolutionary rate. However, COⅠ was not so good DNA barcoding in plant as in animal. Thus, ribosome Internal Transcribed Spacer (ITS) and plastid rb￾cL, matK, trnH-psbA sequences were used as DNA barcoding in plant studying. Although it is on the initial phase for DNA barcoding, facing great challenge, more and more studies showed that DNA barcoding can be widely used in life taxonomy and identification. DNA barcoding, a simple effective accurate species identification method, is now one of the fastest development discipline hot in biological study. Here, in order to promote devel￾opment of Chinese study in DNA barcoding and taxonomy, we introduced DNA barcoding screening, application, 基金项目：本研究由国家科技支撑计划项目(2009BAB44B00)和海南大学“211 工程”创新人才计划项目共同资助