Small-scale interaction experiments Protein-protein interactions pull-down(GST, Ni affinity, Co-immunoprecipitation) cross-linking more biophysical quantitative: fluorescence, CD calorimetry surface plasmon resonance Protein-DNa interactions mostly by gel sh hift assay Many, many thousands of such experiments have been done and reported in the literature but how do you get the information out? This is hard, and an important problem in modern biology PreBiND is a machine learning application that can extract information about whether two proteins interact from the literature automatically http://www.blueprint.orglproducts/prebind/prebind.html Small-scale experiment are generally the most reliable, though still rife with false negatives and false positivesSmall-scale interaction experiments Protein-protein interactions pull-down (GST, Ni affinity, co-immunoprecipitation) cross-linking more biophysical & quantitative: fluorescence, CD, calorimetry, surface plasmon resonance Protein-DNA interactions mostly by gel shift assay Many, many thousands of such experiments have been done and reported in the literature, but how do you get the information out? This is hard, and an important problem in modern biology. PreBIND is a machine learning application that can extract information about whether two proteins interact from the literature automatically. http://www.blueprint.org/products/prebind/prebind.html Small-scale experiment are generally the most reliable, though still rife with false negatives and false positives