Yeast in liquid culture Incubate at 23 C for 5 h Add mutagen distribute into smaller aliquots Plate out dividual Colonies aliquots Agar Incubate at23°C Temperature-sensitive Replica-plate and for growth; growth at 23 incubate no growth at36° 23°C 36°C A Genetic Screen for Cell cycle Mutants Figure by MIT OCWA Genetic Screen for Cell Cycle Mutants Figure by MIT OCW