Skimmilk(Kjeldahl I) Acidify to pH 4.6, teat at 10°Cx20min fake to 12% ool and acidify to pH 4.6, atural with Precipitate (all proteins) (globulins) Total nil K Proteose peptone N- KjeldahL Noseiprotkin dthogernkjekieldah m n= kjeldahl Il-Kjeldahl Iv Figure 4.4 Scheme for quantifying the principal protein fractions in milkSkimmilk (Kjeldahl I) II Heat at loO°C x 20 min. Make to 12% cool and acidify to pH 4.6, filter trichloroacetic acid, filter I Acidify to pH 4.6, filter Precipitate Filtrate (casein) (non-casein N: serum proteins non-protein N; Kjeldahl 11) Neutralize and saturate with filter MgS04 Precipitate (globulins) Filtrate (albumin and non-protein N; Kjeldahl V) Total nitrogen = Kjeldahl I Casein = Kjeldahl I - Kjeldahl II Non-protein nitrogen = Kjeldahl 111 Precipitate Filtrate (casein and (proteose peptone heat-labile and non-protein N; semm proteins) Kjeldahl IV) I Precipitate (all proteins) 1 Filtrate (non-protein nitmgen; Kjeldahl 111) Proteose peptone N = Kjeldahl IV-Kjeldahl III Serum protein = Kjeldahl 11- Kjeldalil IV Figure 4.4 Scheme for quantifying the principal protein fractions in milk