2. Preparation of tissue for LM The most routine one is paraffin section stained with hematoxylin and eosin (H&E The steps a. obtaining th specimen: fresh, small pieces (less than 5mm3)tissue block b Fixation fixatives: use formalin or bouin 's to preserve structural organisation C. Dehydration: use ethyl alcohol to get rid of water of tissue and cell2. Preparation of tissue for LM The most routine one is paraffin section stained with hematoxylin and eosin(H&E) The steps: a. Obtaining th specimen: fresh, small pieces ( less than 5mm3 )-tissue block b. Fixation: fixatives: use formalin or Bouin’s to preserve structural organisation c. Dehydration: use ethyl alcohol to get rid of water of tissue and cell