month of bottle. Park the bottle with paper. Autoclave at 20 lbs for lhr.(将10x2x1厘米的松木条浸泡在1-2%的蔗糖水中。使木条吸足糖水, 装瓶不宜过紧,用纸包好,20磅灭菌1小时。) 2. Pine sawdust medium Pine sawdust(松木屑)75% Rice bran(米糠)23-24% Sucrose(蔗糖)1-2% Water( appropriate)水(适量 Autoclave at20 lbs for 1 hr.(20磅灭菌1小时) 23. Rice straw. Dung Medium(稻草、马粪培养基 Dried horse dung( or cow dung)(干马粪或牛粪)65% Died straw(干稻草)35%Urea(尿素)0.5% Calcium superhosphate(过磷酸钙)0.7% Gypsum powder(石膏粉)1% Ammonium sulphate(硫酸铵)0.3% [Note]: Add appropriate water, pile them up and cause them fermentation (水适量进行堆积发酵) 24. Straw rice bran medium(稻草、米糠培养基) Dried straw(干稻草)75-80% Rice bran(米糠)20-25% [Note]: Select no mouldy straw and cut them about 3 cm in length. Immerse them in fresh water for 12 hr. Allow the straw fully (the amount of water added may be decided by kneading with fingers. It is right when a drol of water appeared between fingers).([注]:选无霉稻草,切成长约3厘米 的小段,用清水浸泡12小时,使充分吸水,然后倾去水,加米糠和水,水量以 用手捏时指间有水珠为宜。) 25. Rice medium(米饭培养基) Fill 10g of rice into a 50ml of Erlenmeyer flask. Add 20-30ml wate Autoclave at 121C for 30 minutes (or fill 2g of rice into a 15 X 150mm tube).Add6 ml of water. Autoclave at12l℃for30 minutes.(取大米 10克,入50毫升椎形瓶中,水20-30毫升于15磅灭菌30分钟即成,或取大米 2克,装入15ⅹ150毫米试管中,加水6毫升,于15磅灭菌30分钟。) 26. Filter Paper Medium(滤纸条培养基) (NH4)2S04 1g KH2P04 1g MgS04.7H200.5 g Yeast Extract(酵母膏)0.1g Distilled Water(蒸馏水)1000ml Strip of filter paper1x7cm(滤纸条1x7厘米) [Note]: Siddolve the above ingredients. Distribute the liqid medium into tubes (each tube contains Iml of the broth). Autoclave at 121C for 30 minutes. Then put one or two sterilized strips of filter paper into a tube aseptically.(将上述溶液分配成溶液后分装入试管,每管1毫升,15磅灭菌 30分钟。再将灭菌的滤纸条(1条或2条)投入试管中即好使用。) 27. Yeast Extract- Medium Extract Agar I(酵母膏、麦芽膏琼脂)month of bottle . Park the bottle with paper . Autoclave at 20 lbs for 1 hr . (将 10ⅹ2ⅹ1 厘米的松木条浸泡在 1-2%的蔗糖水中。使木条吸足糖水， 装瓶不宜过紧，用纸包好，20 磅灭菌 1 小时。) 2. Pine Sawdust Medium Pine Sawdust (松木屑) 75% Rice bran (米糠) 23-24% Socrose (蔗糖) 1-2% Water (appropriate)水 （适量） Autoclave at 20 lbs for 1 hr.(20 磅灭菌 1 小时） 23. Rice Straw. Dung Medium (稻草、马粪培养基) Dried horse dung (or cow dung) （干马粪或牛粪） 65% Died straw （干稻草） 35% Urea （尿素） 0.5% Calcium superhosphate （过磷酸钙）0.7% Gypsum powder（石膏粉） 1% Ammonium sulphate （硫酸铵） 0.3% [Note]: Add appropriate water, pile them up and cause them fermentation. （水适量进行堆积发酵） 24. Straw Rice Bran Medium （稻草、米糠培养基） Dried straw （干稻草） 75-80% Rice bran （米糠） 20-25% [Note]：Select no mouldy straw and cut them about 3 cm in length. Immerse them in fresh water for 12 hr. Allow the straw fully (the amount of water added may be decided by kneading with fingers. It is right when a drop of water appeared between fingers). ( [注]：选无霉稻草，切成长约 3 厘米 的小段，用清水浸泡 12 小时，使充分吸水，然后倾去水，加米糠和水，水量以 用手捏时指间有水珠为宜。) 25. Rice Medium (米饭培养基) Fill 10g of rice into a 50ml of Erlenmeyer flask. Add 20-30ml water. Autoclave at 121℃ for 30 minutes (or fill 2g of rice into a 15ⅹ150mm tube). Add 6 ml of water. Autoclave at 121℃ for 30 minutes. (取大米 10 克，入 50 毫升椎形瓶中，水 20-30 毫升于 15 磅灭菌 30 分钟即成，或取大米 2 克，装入 15ⅹ150 毫米试管中，加水 6 毫升，于 15 磅灭菌 30 分钟。) 26. Filter Paper Medium (滤纸条培养基) (NH4)2SO4 1g KH2PO4 1g MgSO4.7H2O 0.5g Yeast Extract (酵母膏) 0.1g Distilled Water (蒸馏水) 1000ml Strip of filter paper 1ⅹ7 cm (滤纸条 1ⅹ7 厘米) [Note]：Siddolve the above ingredients. Distribute the liqid medium into tubes (each tube contains 1ml of the broth). Autoclave at 121℃ for 30 minutes. Then put one or two sterilized strips of filter paper into a tube aseptically. (将上述溶液分配成溶液后分装入试管，每管 1 毫升，15 磅灭菌 30 分钟。再将灭菌的滤纸条（1 条或 2 条）投入试管中即好使用。) 27. Yeast Extract-Medium Extract Agar I (酵母膏、麦芽膏琼脂)