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Name ection: Question 4 Shown below is a diagram of a replication fork in a double-stranded (ds) DNA molecule found in a prokaryotic cell. Each DNA strand(a and B)serves as a template for polymerization by DNA polymerase, resulting in the formation of a newly synthesized"daughter"DNA strand strand a Replication fork in this 5′ i)On which template strand (A or B)would there be continuous replication by dNA polymerase? What is this newly synthesized daughter strand called during DNA replication? olymerase? What is this newly synthesized daughter strand called during DNA NA ii)On which template strand (a or B)would there be discontinous replication b replication? i)Chemicals that inhibit the enzyme dNa ligase will primarily affect synthesis on one of the two template strands(a or B). Explain on which template strand (a or B) polymerization will be primarily affected and why this occurs b) There are inaccuracies in the dna molecule shown below 5AGTCCGAUGC3 i) Name three things that are wrong in the above DNA sequence ii)What type of chemical interaction is indicated by a" I"in the above diagram? What happens to these interactions during DNA replication? 7012Fall2003Name:___________________________________ Section:_____ 7.012 Fall 2003 Question 4 Shown below is a diagram of a replication fork in a double-stranded (ds) DNA molecule found in a prokaryotic cell. Each DNA strand (A and B) serves as a template for polymerization by DNA polymerase, resulting in the formation of a newly synthesized "daughter" DNA strand. Replication fork moves in this direction 5’ 3’ 3’ 5’ strand A strand B a) i) On which template strand (A or B) would there be continuous replication by DNA polymerase? What is this newly synthesized daughter strand called during DNA replication? ii) On which template strand (A or B) would there be discontinous replication by DNA polymerase? What is this newly synthesized daughter strand called during DNA replication? iii) Chemicals that inhibit the enzyme DNA ligase will primarily affect synthesis on one of the two template strands (A or B). Explain on which template strand (A or B) polymerization will be primarily affected and why this occurs. b) There are inaccuracies in the DNA molecule shown below. 1 5 10 5' A G T C C G A U G C 3' | | | | | | | | | | 5' T C A G G C T A T G 3' i) Name three things that are wrong in the above DNA sequence. ii) What type of chemical interaction is indicated by a "|" in the above diagram? What happens to these interactions during DNA replication?
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