Primary structure Sequencing Nucleic acids核酸序列测定 Chain termination method(dideoxy method) developed by f. sanger Base-specific chemical cleavage, developed by Maxam and gilbert Both use autoradiography -X-ray film develops in response to presence of radioactive isotopes in nucleic acid moleculesPrimary Structure Sequencing Nucleic Acids 核酸序列测定 • Chain termination method (dideoxy method), developed by F. Sanger • Base-specific chemical cleavage, developed by Maxam and Gilbert • Both use autoradiography - X-ray film develops in response to presence of radioactive isotopes in nucleic acid molecules