PCR技术诞生的时代背景 H. Ghobind Khorana及其同事(1971) 经过DNA变性,与合适的引物杂交,用DNA聚合酶延伸引物,并不断重复 该过程便可克隆RNA基因。 迅速被遗忘 Dr Khorana, then at the University of wisconsin, shared the 1968 Nobel in physiology or medicine with Robert w. Holley of Cornell University and Marshall w. Nirenberg of the National Institutes of Health They worked independently and won the award "for their interpretation of the genetic code and its function in protein synthesis, according to the Nobel citation.PCR技术诞生的时代背景 H. Ghobind Khorana及其同事（1971）： 经过DNA变性，与合适的引物杂交，用DNA聚合酶延伸引物，并不断重复 该过程便可克隆tRNA基因。 迅速被遗忘…… Dr. Khorana, then at the University of Wisconsin, shared the 1968 Nobel in physiology or medicine with Robert W. Holley of Cornell University and Marshall W. Nirenberg of the National Institutes of Health. They worked independently and won the award “for their interpretation of the genetic code and its function in protein synthesis,” according to the Nobel citation