Step 1 Flood the heat-fixed smear with crystal violet for 1 min 结晶紫染1分钟 All cells purple Step 2 Add iodine solution for 3 min 碘液媒染3分钟 All cells remain purple Steps in the Gram stain procedure.革兰氏染色过程Steps in the Gram stain procedure.革兰氏染色过程 结晶紫染1分钟 碘液媒染3分钟