BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 Larger igands are always prevented from accessing the pocket next to the immobilized chain ctive access occurs for the second binding pocket 20 a away- when the chain is collapsed it does not prevent access to the second pocket, but when hydrated, long chains can prevent ccess to the neighboring pocket and block protein binding Mechanisms of Biotin Smart switch operation Blocked T>LCST T< LCST Accessible Fabrication of capture and release devices o Conjugation to magnetic micro-or nano-spheres Affinity purification Affinity purification Responsive drug release Conjugation to magnetic microspheres/nanospheres Generality of concept o Switch temperature can be tuned by copolymerizing with more hydrophilic monomers such as hydroxyethyl methacrylate Lecture 13-Hybrid macromolecules 4 of 13B B BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 o Larger protein ligands are always prevented from accessing the pocket next to the immobilized chain ƒ Selective access occurs for the second binding pocket 20 Å away- when the chain is collapsed it does not prevent access to the second pocket, but when hydrated, long chains can prevent Mechanisms of switch operation: access to the neighboring pocket and block protein binding ƒ Fabrication of capture and release devices3,5 o Conjugation to magnetic micro- or nano-spheres ƒ Affinity purification QuickTime™ and a Graphics decompressor are needed to see this picture. QuickTime™ and a Graphics decompressor are needed to see this picture. QuickTime™ and a Graphics decompressor are needed to see this picture. QuickTime™ and a Graphics decompressor are needed to see this picture. QuickTime™ and a Graphics decompressor are needed to see this picture. QuickTime™ and a Graphics decompressor are needed to see this picture. QuickTime™ and a Graphics decompressor are needed to see this picture. QuickTime™ and a Graphics decompressor are needed to see this picture. QuickTime™ and a Graphics decompressor are needed to see this picture. QuickTime™ and a Graphics decompressor are needed to see this picture. Applications: • Affinity purification 1) QuickTime™ and a Graphics decompressor are needed to see this picture. QuickTime™ and a Graphics decompressor are needed to see this picture. QuickTime™ and a Graphics decompressor are needed to see this picture. 3) B B QuickTime™ and a Graphics decompressor are needed to see this picture. B B B B Conjugation to magnetic microspheres/nanospheres B B B 2) B B B B • Cell-surface labeling • Responsive drug release QuickTime™ and a Graphics decompressor are needed to see this picture. QuickTime™ and a Graphics decompressor are needed to see this picture. QuickTime™ and a Graphics decompressor are needed to see this picture. QuickTime™ and a Graphics decompressor are needed to see this picture. QuickTime™ and a Graphics decompressor are needed to see this picture. QuickTime™ and a Graphics decompressor are needed to see this picture. QuickTime™ and a Graphics decompressor are needed to see this picture. QuickTime™ and a Graphics decompressor are needed to see this picture. QuickTime™ and a Graphics decompressor are needed to see this picture. QuickTime™ and a Graphics decompressor are needed to see this picture. ƒ Generality of concept o Switch temperature can be tuned by copolymerizing with more hydrophilic monomers such as hydroxyethyl methacrylate Lecture 13 – Hybrid macromolecules 4 of 13