Gene from Pseudomonas fluorescens BlT-18 JIANG Fang-yan, HUANG Shen, LI Chun' (School of Life Science, Beijing Institute of Technology, Beijing 100081, China; 2School of Biological Science and Technology, Qiongzhou University, Sanya Hainan, 572022 China Abstract: a gene from Pseudomonas fluorescens BIT-18 encoding a protein with phospholipase B activity(Pf-PLB) was cloned and sequenced. The bioinformatics analysis results showed that Pf-PLB containing an open reading frame 1272 bp in length. The cloned gene was predicted to encode a protein of 423 amino acid residues with a theoretical molecular mass of 45.8 kDa and a deduced pl of 5.53. According to the signal peptide prediction by the SignalP, a putative signal peptide cleavage site was situated at 23 amino acids after the initiation codon. Phylogenetic and sequence analy sis revealed that pfplb is a member of a new gene family of PLBs. The homology modeling by Modeller exhibited that Pf-PLB was a B-barrel protein which consisted of 14 strands. This research laid the foundations for the development of more effective expression systems and the degumming mechanism of Pf-PLB Keywords: aza-crown ether, nicotinic acid; crystal structure 参考文献录入示样: 1期刊论文J 5] De Maria L, Vind J, Patkar S, et al. Phospholipases and their industrial applications [J]Applied Microbiology Biotechnology, 2007, 74(2): 290-300 6]姜芳燕产磷脂酶菌株的筛选鉴定及其应用[化工学报,2012,63(3):887-893 [7 Hearn EM, Patel DR, Lepore Bw, et al. Transmembrane passage of hydrophobic compounds through a protein channel wall [] Nature, 2009, 458: 367-370 [8]梁成伟,程江峰,王帅,等八氢番茄红素脱氢酶(PDS)基因分子进化特征叩青岛科技大学学报(自然 科学版,2009(5):408-411 2专著: [6]万哲先代数导引[M].北京:科学出版社,2004 [7]海德格尔.在通向语言的途中[M].孙周兴,译.北京:商务印书馆,2005 3.论文集中析出文献:4 Gene from Pseudomonas fluorescens BIT-18 JIANG Fang-yan1,2 , HUANG Shen1 , LI Chun1 ( 1School of Life Science, Beijing Institute of Technology, Beijing 100081, China; 2School of Biological Science and Technology, Qiongzhou University, Sanya Hainan, 572022, China) Abstract: A gene from Pseudomonas fluorescens BIT-18 encoding a protein with phospholipase B activity (Pf-PLB) was cloned and sequenced. The bioinformatics analysis results showed that Pf-PLB containing an open reading frame 1272 bp in length. The cloned gene was predicted to encode a protein of 423 amino acid residues with a theoretical molecular mass of 45.8 kDa and a deduced pI of 5.53. According to the signal peptide prediction by the SignalP, a putative signal peptide cleavage site was situated at 23 amino acids after the initiation codon. Phylogenetic and sequence analysis revealed that pfplb is a member of a new gene family of PLBs. The homology modeling by Modeller exhibited that Pf-PLB was a β-barrel protein which consisted of 14 strands. This research laid the foundations for the development of more effective expression systems and the degumming mechanism of Pf-PLB. Keywords：aza-crown ether; nicotinic acid; crystal structure 参考文献[录入示样]： 1.期刊论文[J] [5] De Maria L, Vind J, Patkar S, et al. Phospholipases and their industrial applications [J].Applied Microbiology Biotechnology, 2007, 74(2): 290-300. [6] 姜芳燕.产磷脂酶菌株的筛选鉴定及其应用[J].化工学报, 2012, 63(3): 887-893. [7] Hearn EM, Patel DR, Lepore BW, et al. Transmembrane passage of hydrophobic compounds through a protein channel wall [J]. Nature, 2009,458: 367-370. [8] 梁成伟, 程江峰, 王帅, 等.八氢番茄红素脱氢酶（PDS）基因分子进化特征[J]. 青岛科技大学学报(自然 科学版), 2009(5): 408-411. 2.专著: [6]万哲先.代数导引[M].北京:科学出版社,2004. [7]海德格尔.在通向语言的途中[M].孙周兴,译.北京:商务印书馆,2005. 3.论文集中析出文献: