C. Liu et al/ Journal of Luminescence 119-120(2006)132-136 1.41280cm B-sheet structural absorptions in malignant tissue but smaller in normal and benign tissues [3]. The double peak at 1464 and 1474 cm can be clearly 巴 1.0 observed in sr-ftir but not in conventional ir experiments, because the resolving power is low in the latter method and the double peak cannot be tissues, the relative high intensity of two reflects the metabolizing is strong in malignant tumor tissues. Cancer cells have a higher meta bo- 2800 3000 3200 3400 3600 lism than normal, healthy cells. The peak of Wavenumber(cm) 1500 cm. which is related to the c=c vibration of pyrrole in red cells, is usually weaker in tissues 1.0x10 and difficult to be observed. This peak exists only in spectrum of malignant tissue. It is probably due to the of red cells in process of vascul genesis in breast tissues during their cancerous progress [11]. It might be an important aura of -5.0×105 reas cancer fact that 3100-3500cm region are not apparent in tumor 1.0X10 and cancer tissues reflects certain kinds of sub structures inside the N-h bond are gradually 1.5x1 damaged in progression to cancer. Wavenumber(cm") Fig. 3. IR absorption spectra in the lipids and N-H bond ption region (a) and their order derivatives (b) 4. Conclusions within 3100-3500cm lipids absorptions and ertain sub-structures relative to H bond are observed Curves I, II and Ill represent abs In summary, by synchrotron radiation based malignant tissues, respectively. FTiR absorption spectra, there are obvious differences of spectral structures among benign and malignant breast tissues. Having analyzed carefully the whole IR absorption groups are increased in the progression to cancer. spectrum in energy range of 900-3600 cm,we The complicated spectrum structures could have a general impression: the Ir absorption understood by the fact that in malignant tissues a spectrum varies from to be featured abundantly to big portion of chemical bonds of proteins is faint from normal breast tissue to benign tumors roken in cancerous progress. The shifts of while it develops from relatively smooth spectrum absorption peaks maybe result from such kind of to much more complicated one in progression to deterioration and the putrescence of tissues as well cancer of the diseased tissue. on the other hand The 968 cm"peak observed only in benign tissues the high resolving power of synchrotron radiation shows the splitting of cells or/ and dNA in growing based FTIR can make closer peaks, such as the process of tumor [10). The peak of 1655 cm is double peak at 1464 and 1474 cm be visible. attributed to the absorption of the C=O stretch- Some specific absorption peaks are found by ing vibration coupled to the in-phase bending of synchrotron radiation based FTI the N-H bond which may represent certain kind of may help us diagnose whether the breast tissue is a helix structure inside [7]. The peak at 1543 cm healthy or diseased, or in which stage of progres may represent that there is larger anti-parallel Sion to cancersgroups are increased in the progression to cancer. The complicated spectrum structures could be understood by the fact that in malignant tissues a big portion of chemical bonds of proteins is broken in cancerous progress. The shifts of absorption peaks maybe result from such kind of deterioration and the putrescence of tissues as well. The 968 cm
1 peak observed only in benign tissues shows the splitting of cells or/and DNA in growing process of tumor [10]. The peak of 1655 cm
1 is attributed to the absorption of the CQO stretch￾ing vibration coupled to the in-phase bending of the N–H bond which may represent certain kind of a helix structure inside [7]. The peak at 1543 cm
1 may represent that there is larger anti-parallel b-sheet structural absorptions in malignant tissue but smaller in normal and benign tissues [3]. The double peak at 1464 and 1474 cm
1 can be clearly observed in SR–FTIR but not in conventional IR experiments, because the resolving power is low in the latter method and the double peak cannot be resolved. Compare with normal and benign tissues, the relative high intensity of two peaks reflects the metabolizing is strong in malignant tumor tissues. Cancer cells have a higher metabo￾lism than normal, healthy cells. The peak of 1500 cm
1 , which is related to the CQC vibration of pyrrole in red cells, is usually weaker in tissues and difficult to be observed. This peak exists only in spectrum of malignant tissue. It is probably due to the increase of red cells in process of vasculo￾genesis in breast tissues during their cancerous progress [11]. It might be an important aura of breast cancer. The fact that all peaks at 3100–3500 cm
1 region are not apparent in tumor and cancer tissues reflects certain kinds of sub￾structures inside the N–H bond are gradually damaged in progression to cancer. 4. Conclusions In summary, by synchrotron radiation based FTIR absorption spectra, there are obvious differences of spectral structures among normal, benign and malignant breast tissues. Having analyzed carefully the whole IR absorption spectrum in energy range of 900–3600 cm
1 , we have a general impression: the IR absorption spectrum varies from to be featured abundantly to faint from normal breast tissue to benign tumors; while it develops from relatively smooth spectrum to much more complicated one in progression to cancer of the diseased tissue. On the other hand, the high resolving power of synchrotron radiation based FTIR can make closer peaks, such as the double peak at 1464 and 1474 cm
1 be visible. Some specific absorption peaks are found by synchrotron radiation based FTIR method, which may help us diagnose whether the breast tissue is healthy or diseased, or in which stage of progres￾sion to cancers. ARTICLE IN PRESS 2800 3000 3200 3400 3600 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 Absorbance (a.u.) Wavenumber (cm-1) III II I 2955cm-1 2917cm 2850cm -1 -1 3100 3200 3300 3400 3500 -1.5x10-4 -1.0x10-4 -5.0x10-5 0.0 5.0x10-5 1.0x10-4 Second Order Derivative Wavenumber (cm-1) III II 3282cm-1 3198cm-1 I 3426cm-1 3488cm-1 (a) (b) Fig. 3. IR absorption spectra in the lipids and N–H bond absorption region (a) and their second order derivatives (b) within 3100–3500 cm
1 region. Strong lipids absorptions and certain sub-structures relative to the N–H bond are observed. Curves I, II and III represent absorption of normal, benign and malignant tissues, respectively. C. Liu et al. / Journal of Luminescence 119– 120 (2006) 132–136 135