3. Codons for initiation of translation Major codon for initiation iS AUG Regardless of codon used the first amino acid incorporated in E. coli is formyl-met For the 4288 genes identified in E. coli AUG is used for 3542 genes GUG is used for 612 genes UUG is used for 130 genes AUU is used for 1 gene CUG may be used for 1 gene3. Codons for initiation of translation • Major codon for initiation is AUG • Regardless of codon used, the first amino acid incorporated in E. coli is formyl-Met. • For the 4288 genes identified in E. coli: AUG is used for 3542 genes. GUG is used for 612 genes. UUG is used for 130 genes. AUU is used for 1 gene. CUG may be used for 1 gene