Eur J Plant Pathol (2012)133:899-910 909 in R.similis (with dsRNA Fallas,G.A Sarah,J.L.(199).Effect ture on the in vitro uction of xyl/clearly A.Xu.S.0.M 175-177 the expr M.K Kostas S A Driv Rs-cb-1)wa S.E.Mel is in dsR of Rs 391,806-811 was obtained for 48 F.M.Klap.C.& study, of Rs-eng-1b and treatments for 36 h had the 863-873. eng- ble pm-e Acknowledgments This work was funded by National Natu or Agr-Scimufe Re Public) cs of b c,R.A.Sikor (Ea bution of vector PYL32-d1-GFPn. References Bakhetia,M.,Urwin, P.E..Atkins H..(2007) Hacg Ha eman.A..Vanholme.B.&Gheysen.G.(2009).Chara .T..Allen.R.Davis. & ...Smant,.Jones,J.T.(2005).Func Hussey.R.S.(04).Use of solid tional analysis of patho roteins o atod era ros Jacob.Vanholme.B.Hacgeman.&Gheysen.G( ar sitic nematode s of an 12.225-233. 280.1-17 Jon Eds.), 3607)UK: BS.Lau.Y. .Chenchik.A. MCRA Gurskaya.N.Sverdlov,E.D..&Siebert,P.D.(1999 e through ingested double-stranded ome Bic in Enzymology:303,349-380 from http://genomebiology.com/2000/2/1/research/0002. Springe endoxylanase in R. similis (Rs-xyl1) with dsRNA for 24 h and the expression of Rs-xyl1 clearly reduced. Li et al. (2010) reported that the expres￾sion of the Cathepsin B in R. similis (Rs-cb-1) was clearly reduced after soaking R. similis in dsRNA of Rs-cb-1 for 24 h, and the best silencing effect was obtained after RNAi treatment for 48 h. In this study, expression of Rs-eng-1b decreased signifi￾cantly after soaking R. similis in dsRNA of Rs￾eng-1b for 24 h, and treatments for 36 h had the best effect overall. Acknowledgments This work was funded by National Natu￾ral Science Foundation of China (31071665) and Special Fund for Agro-Scientific Research in the Public Interest (200903040). The support of Fuliang Xie (East Carolina University, USA) is gratefully acknowledged. 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