Phase-Contrast Microscopy Eye Ocular lens Diffraction plate Undiffracted light (unaltered by specimen) Objective lens Refracted or diffracted light(altered by specimen) Accentuates diffraction of Specimen Condenser lens the light that passes through a specimen. Annular diaphragm Light (c)Phase-contrast Figure 3.4c • Accentuates diffraction of the light that passes through a specimen. Phase-Contrast Microscopy Figure 3.4c