0.50.50.50.50.50.5 50.50.50.5 Serum dilution1:101:201:401801:1601:3201:6401:12801:2560 Serum 05ml 1:1280 1:5120 dilution 1:40 1:160 1:640 1:2560 control10 10 Order 1 2 3 4 5 6 7 8 9 10 Serum 0.5ml Discard 0.5ml Saline 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Serum dilution 1:10 1:20 1:40 180 1:160 1:320 1:640 1:1280 1:2560 Final 1:20 1:80 1:320 1:1280 1:5120 dilution 1:40 1:160 1:640 1:2560 control