Differential media Triple Sugar Iron(TSD slant Ingredients glucose sucrose, lactose, Slant, lactose, FeSO4 ferrous sulfate, and pH indicator phenol red (phenol red); Bacteria that ferment any of the three Butt, glucose, phenol red sugars in the medium will produce byproducts. These byproducts are usually acids, which will change the color of the ph indicator from red to yellow; Some bacteria utilize thiosulfate anion as a terminal electron acceptor, reducing it to hydrogen sulfide. The byproduct H2s reacts with ferrous sulfate in the medium to form black precipitate. E Coli Samonella ShigellaDifferential media Triple Sugar Iron (TSI) slant • Ingredients : glucose ,sucrose , lactose , ferrous sulfate, and pH indicator (phenol red); • Bacteria that ferment any of the three sugars in the medium will produce byproducts. These byproducts are usually acids, which will change the color of the pH indicator from red to yellow; • Some bacteria utilize thiosulfate anion as a terminal electron acceptor, reducing it to hydrogen sulfide. The byproduct H2S reacts with ferrous sulfate in the medium to form black precipitate. Slant, lactose,FeSO4, phenol red Butt, glucose, phenol red E.Coli Samonella Shigella