TABLE 4.2 Commercially Available RNA lsolation Reagents Chemistry Kit Name Manufacturer Starting Material Yield Kits available agnetic beads ChargeSwitch total RNA Invitrogen Up to 25ug from 106 cells EZI RNA Tissue kit Qiagen 10-40g5-10 mg tissue MagAttract RNA kit Qiagen 5,7 5-20ug RNA per 1 x 10 cultured cells MagMAX Blood kit Ambion 1,4 300-500 ng from 50uL of whole blood MagNA Pure total rNa Roche diagnostic 1,3-5,7 MagneSil Total RNA Promega 1,5,7 2ug from 10 cells RNAdvance cell kit Agencourt Beckmann Coulter 5 Iug from 10 cells RNAgents Total rna isolation 1,2,5.7,8 1.3 mg from 10 WBC blood Tri reagent Sigma Aldrich 1,3-5,7 5-15ug from 10 cells TRIzol RNA purification Invitrogen 1,3,5,.7,8 Solid-phase adsorption Gen Elute Mammalian total RNA Sigma Aldrich Up to 150ug from 10/ cells Purelink Total RNA kit Invitrogen 1,5,7,8 I mg from 10 cells QIAamp RNA kit Qiagen 1,7 1-5ug rna per ml of whole blood Recover ALL Total Nucleic Acid Ambion RiboPure kit Ambion 1,5,7 2-4ug/0.5 mL whole blood SV Total RNA Isolation Promega 2,5,7 20-30ug(1.2 cm tissue) Sample Types: whole blood (1), buffy coat(2), plasma 3), body fluids(4), cultured cells 5), cell suspension (6), tissue homogenates () bacteria(8), bone marrow (9), buccal cells(10), paraffin-fixed