Histogram of 5'ss Scores 2000 PseudO-n。 eco Teon。2s True 1500 15 5 Splice 1000 1。 Splice Sites soo so Sites 200 150 100 Score(1/10 bit units) Measuring accuracy Sn:20%50%90% Sensitivity = of true sites w/score> cutoff Specificity = of sites w/ score cutoff sp:50%32%7% that are true sitesHistogram of 5’ss Scores True 5’ Splice Sites “Decoy” 5’ Splice Sites Score (1/10 bit units) Measuring Accuracy: Sensitivity = % of true sites w/ score > cutoff Specificity = % of sites w/ score > cutoff that are true sites Sn: 20% 50% 90% Sp: 50% 32% 7%