2. Pyruvate is oxidized to acetyl- CoA by the catalysis of pyruvate R dehydrogenase complex Pyruvate is first transported into mitochondria via a specific transporter on the inner membrane Pyruvate is converted to acetyl-COA and CO2 by oxidative decarboxylation. DI The pyruvate dehydrogenase complex is a huge multimeric assembly of three kinds of enzymes having 60 subunits in bacteria and more in mammals Pyruvate is first decarboxylated after binding to the prosthetic group (TPP)of pyruvate dehydrogenase (E), forming hydroxyethyl-TPP7073743292. Pyruvate is oxidized to acetyl￾CoA by the catalysis of pyruvate dehydrogenase complex • Pyruvate is first transported into mitochondria via a specific transporter on the inner membrane. • Pyruvate is converted to acetyl-CoA and CO2 by oxidative decarboxylation. • The pyruvate dehydrogenase complex is a huge multimeric assembly of three kinds of enzymes, having 60 subunits in bacteria and more in mammals. • Pyruvate is first decarboxylated after binding to the prosthetic group (TPP) of pyruvate dehydrogenase (E1 ), forming hydroxyethyl-TPP