Temperature A most important factor influencing the growth is the temperature sensitivity of enzyme catalyzed reactions High temperature 1. Denature enzymes transport carries and other proteins 2. Microbial membranes are also disrupted by temperature extremes 3. The lipid bilayer melts and disintegratesTemperature • A most important factor influencing the growth is the temperature sensitivity of enzyme catalyzed reactions. • High temperature: 1. Denature enzymes, transport carries, and other proteins. 2. Microbial membranes are also disrupted by temperature extremes. 3. The lipid bilayer melts and disintegrates