Bacterium ①Vector such as a DNA is cleaved DNA containing by an enzyme plasmid is isolated gene of interest into fragments 3 Gene is inserted Bacterial Plasmid into plasmid chromosome Recombinant DNA Gene of (plasmid) interest Plasmid is taken up by a cell such as a bacterium Recombinant bacterium ⑤Cells with gene of interest are cloned Figure 9.1.1Figure 9.1.1