DAIRY CHEMISTRY AND BIOCHEMISTRY Fraction number WCx1 X VIII VII VI V IV IⅡ 触学 Figure 4.5(a) Chromatogram of sodium caseinate n column of deae cellul anion exchanger. Buffer: 5 M urea in imidazole-HCI pH 7.0; gradient: 0-0.5 M NaCl. of sodium caseinate on a Pharmacia Mono Q HRS/5 anion exchange column Buffer: 6 M in 5 mM bis-tris-propane7 mM HCl, pH 7; gradient: 0-0.5 M NaCl. (d) Chromatogram of sodium caseinate on a Pharmacia Mono S Hr5/5 cation exchange column Buer: 8 M urea in 20 mM acetate buffer pH 5: gradient: 0-1.0 M NaCl156 DAIRY CHEMISTRY AND BIOCHEMISTRY N X L Y &- 20 40 00 80 LOO I20 I40 160 180 2w 220 Fraction number Figure 4.5 (a) Chromatogram of sodium caseinate on an open column of DEAE cellulose anion exchanger. Buffer: S M urea in imidazole-HCI buffer, pH 7.0; gradient: 0-0.5 M NaC1. (b) Urea polyacrylamide gel electrophoretograms of the fractions from (a). (c) Chromatogram of sodium caseinate on a Pharmacia Mono Q HR5/S anion exchange column. Buffer: 6 M urea in 5 mM bis-tris-propane/7 mM HCI, pH 7; gradient: 0-0.5 M NaC1. (d) Chromatogram of sodium caseinate on a Pharmacia Mono S HRS/S cation exchange column. Buffer: 8 M urea in 20 mM acetate buffer, pH 5; gradient: 0-1.0 M NaC1