Fungal immunomodulatory proteins 5 could be increased to 800 mg/L (Lin et al.,2009a).The FIP-fie the natural one.The circular dichroism analysis result indicated gene is cloned from the genome DNA of F velutipes.The that the relative proportion of the secondary structure units were recombinant expression vectors pET-28(+)-FIP-fve is reconstructed similar to the natural FIB which might mean that the similar nature and transformed into GS115 strain.The effective transformants and bioactive.reFIP-fve were correctly folded and formed.And are obtained and the yield of recombinant FIP-fve is about 152 the phenol-sulfuric acid method showed that the sugar content of mg/L (Xu,2009).The amino acid composition analysis showed the reFIP-fve was 4.2%indicating the occurrence of glycosylation that the recombinant FIP had the same amino acid composition as of reFIP in the yeast expression system (Xu,2009) FIGURE 5.Schematic representation of the affecting of immunomodulatory macrofungi metabolites on the adaptive immune system leading to activation of antimicrobial and antitumor pathways.(Jeurinka et al.,2008).Ag,antigen;PPCs,polysaccharide peptide/ protein complexes;FIPs,fungal immunomodulatory proteins;APC,antigen presenting cell;TLR,toll like receptor;CRI,complement receptor 1;AC,activated complement;NF-KB,nuclear factor kappa B;CD,cluster designation;MHC II,major histocompatibility complex;TCR,T-cell receptor;NO,nitric oxide;IL,interleukin;IFN-Y,interferon y LT,lymphotoxin;TH,T helper cell;NK cell,natural killer cell;CTL cytotoxic T lymphocyte. Ag AC B-D-glucans PPCs TLR CRI mushroom FIPs NF-kB L-12 NO Cytokine NK cell MHCII CD28 Target cell dies IFN-Y Synthesis of by apoptosis IgE antibody L-13 CTL Tal TH2 LT Pre-CD8+ L-2 L-4 T-cell IFN-Y IL-10 Neutrophil Opsinzation activation 电 Eosinophil Suppression of mac- activation rophage activation phygocytosis enhanced Mast cell B-cell B-cell IEN- microbial degranulation killing Macrophage IL-4 activation B-cell Neutralizing Antitumor Antimicrobial Cytokine IgG antibody functions functions production Cell-mediate Immunity Inflamnation Humororal-mediate ImmunityFungal immunomodulatory proteins 5 could be increased to 800 mg/L (Lin et al., 2009a). The FIP-fve gene is cloned from the genome DNA of F. velutipes. The recombinant expression vectors pET-28(+)-FIP-fve is reconstructed and transformed into GS115 strain. The effective transformants are obtained and the yield of recombinant FIP-fve is about 152 mg/L (Xu, 2009). The amino acid composition analysis showed that the recombinant FIP had the same amino acid composition as the natural one. The circular dichroism analysis result indicated that the relative proportion of the secondary structure units were similar to the natural FIP, which might mean that the similar nature and bioactive. reFIP-fve were correctly folded and formed. And the phenol-sulfuric acid method showed that the sugar content of the reFIP-fve was 4.2% indicating the occurrence of glycosylation of reFIP in the yeast expression system (Xu, 2009). FIGURE 5. Schematic representation of the affecting of immunomodulatory macrofungi metabolites on the adaptive immune system leading to activation of antimicrobial and antitumor pathways. (Jeurinka et al., 2008). Ag, antigen; PPCs, polysaccharide peptide/ protein complexes; FIPs, fungal immunomodulatory proteins; APC, antigen presenting cell; TLR, toll like receptor; CR1, complement receptor 1; AC, activated complement; NF-κB, nuclear factor kappa B; CD, cluster designation; MHC II, major histocompatibility complex; TCR, T-cell receptor; NO, nitric oxide; IL, interleukin; IFN-γ, interferon γ; LT, lymphotoxin; TH, T helper cell; NK cell, natural killer cell; CTL, cytotoxic T lymphocyte