Primary Interactions Between Antigen and Antibody Determined by the interaction between epitopes and antigen-binding sites Independent of biochemical and biologic phenomena that may follow Depend only on the quantity and quality (affinity/avidity)of antibodies The basis for ELISA,RIA,IFA assaysPrimary Interactions Between Antigen and Antibody ◼ Determined by the interaction between epitopes and antigen-binding sites ◼ Independent of biochemical and biologic phenomena that may follow ◼ Depend only on the quantity and quality (affinity/avidity) of antibodies ◼ The basis for ELISA, RIA, IFA assays