◆1,2-二氧杂环丁烷类 I LOcHa Alkaline Phosphatase HaCO A lkaline phosphatase(ALP)dephosphorylates AMPPD (1,2-二氧杂环丁烷类) to form a dioxetane anion, O-0OCHs which fragments into adamantanone(金刚烷酮)and the excited state of methyl meta- oxybenzoate anion (间氧苯甲酸甲酯阴离子),the| ight emitter AMPPD R=HR2=H The detect limit of alp is 10-21moI/L CSDP: R=CL R2=H CDP-Star:R=CL,R=CI The derivatives of amppd are superior than the parent compound to the lighting rates and signal• Alkaline phosphatase (ALP) dephosphorylates AMPPD (1,2-二氧杂环丁烷类） to form a dioxetane anion, which fragments into adamantanone （金刚烷酮）and the excited state of methyl meta-oxybenzoate anion （间氧苯甲酸甲酯阴离子）, the light emitter. • The detect limit of ALP is 10-21mol/L. • The derivatives of AMPPD are superior than the parent compound to the lighting rates and signal intensities. 1,2-二氧杂环丁烷类