Rate Law in Enzyme Catalyzed Reactions E+SES E+p Rate law still applies in enzyme catalyzed reactions. The forward velocity,or rate,v is, v,=k [EIS] The reverse velocity or rate,or the rate of disappearance va is, va k[ES]+k2[ES]=(k+k2)[ES] At steady state,there is no accumulation of ES],thus: Vf =VdRate Law in Enzyme Catalyzed Reactions • Rate law still applies in enzyme catalyzed reactions. • The forward velocity, or rate, vf is, • The reverse velocity or rate, or the rate of disappearance vd is, • At steady state, there is no accumulation of [ES], thus: E + S ES E + P k1 K-1 k2 v k ES f = 1 v k ES k ES k k ES d ( ) = −1 + 2 = −1 + 2 f d v = v