G C C 34监测蛋白质 T A 350 CGCG 300 G C 250 T A 200 C G 8150 8100 0二P B 5 10 H+4 Time(s) Figure 1.(A) The molecular beacon DNA molecule(MB1).(B) Fluorescence intensity time scan of the MB-binding reaction with LDH- TMR DABCYT 5. Measurements were taken to ensure that diffusion was not a factor in the reaction rate(15 s was required to fully mix the MB and LDH-5 solutions before the measurements). The concentrations of MB and LDH were 0.5 and 1 uM. The buffer used was 20 mM Tris/HCl, 0.05 mM MgCl2, pH=7.83.4 监测蛋白质