Wall-less forms of bacteria A 2873B71e, o When bacteria are treated with 1 )enzymes that are lytic for the cell wall e.g. lysozyme or 2 antibiotics that interfere with biosynthesis of peptidoglycan, wall-less bacteria are often produced o Usually these treatments generate non-viable organisms Wall-less bacteria that can not replicate are referred to as spheroplasts when an outer membrane is present)or protoplasts(if an outer membrane is not present o Occasionally wall-less bacteria that can replicate are generated by these treatments(L forms)Wall-less forms of Bacteria. ⚫ When bacteria are treated with 1) enzymes that are lytic for the cell wall e.g. lysozyme or 2) antibiotics that interfere with biosynthesis of peptidoglycan, wall-less bacteria are often produced. ⚫ Usually these treatments generate non-viable organisms. Wall-less bacteria that can not replicate are referred to as spheroplasts (when an outer membrane is present) or protoplasts (if an outer membrane is not present). ⚫ Occasionally wall-less bacteria that can replicate are generated by these treatments (L forms)