8/30/2016 ●●0 Ribotyping 3 different stains of bacteria Very labor intensive if done manually extract DNA Requires an automated system called 雳雳麗 the Riboprinter tasnanne 米 s23 DNA fragments separated by size transfer DNA to Potpourri of Rapid Direct epifluorescence filter technique Methods -Bacteria are illuminated Bioluminescence-based methods Membrane filtration of a food Provide a rapid estimate of sample to collect cells total microbial numbers Light production correlates Stain bacteria with fluorescent with the number of microbes antibodies Uni-Lite System:results <1 min Visualize using epifluorescent microscope DAPI stained bacterial cells from drinking water8/30/2016 9 genes coding for the 16S d 23S RNA 16S and 23S rRNA Ribotyping Very labor intensive if done manually Requires an automated system called th e Rib i t Riboprinter Potpourri of Rapid Methods Bioluminescence-based methods - Provide a rapid estimate of total microbial numbers - Light production correlates with the number of microbes - Uni-Lite System: results <1 min Direct epifluorescence filter technique - Bacteria are illuminated - Membrane filtration of a food sample to collect cells - Stain bacteria with fluorescent antibodies - Visualize using epifluorescent microscope DAPI stained bacterial cells from drinking water