History Six months after outbreak cdc isolated bacteria from post-mortum lung tissue Inoculated lung tissue into peritoneal cavity of guinea pigs After animal became ill, removed its spleen and injected tissue into fertilized chicken eggs (rickettsial methodology) BIOL 533 Lecture 14BIOL 533 3 Lecture 14 History • Six months after outbreak, CDC isolated bacteria from post-mortum lung tissue – Inoculated lung tissue into peritoneal cavity of guinea pigs – After animal became ill, removed its spleen and injected tissue into fertilized chicken eggs (rickettsial methodology)